Effects of Bavistin and Cefotaxime on in vitro Contaminant free Shoot Regeneration of Ruellia tuberosa L.

In general, antimicrobial agents are often used in micropropagation techniques to obtain contaminant free clones. The objective of the present study was evaluate effects bavistin and cefotaxime on producing plants Ruellia tuberosa cultured MS supplemented with phytohormones. Field grown nodal explants regenerate entire via direct organogenesis. Among decontaminants tested, fungicide along higher concentration BAP (2.0 mg/l) lower NAA (1.0 most effective regeneration shoots from explants. This at 300 mg/l minimised fungal contamination survival rate 54%. While addition decontaminant low (200 same stimulated bud formation controlled bacterial contamination. However, its increasing adversely affected tuberosa. These findings clearly showed that concentrations were not only non-toxic but also facilitated regeneration. results achieved decisive role use successful fungicides antibiotics, their sufficient doses very important reducing helping multiple shoot proliferation.
 Plant Tissue Cult. & Biotech. 31(1): 1-12, 2021 (June)